FastQCFastQC Report
Thu 2 Jun 2016
DRR028546_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028546_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3715330
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC47250.051.277484
ATCAACG48300.050.616033
GGTATCA34450.046.815911
GTATCAA55500.045.1015551
CAACGCA56450.043.0473985
AACGCAG60250.040.3323756
TATCAAC63800.038.433422
ACGCAGA65550.036.8476687
GTACATG91050.035.9879231
CGCAGAG66050.035.570528
TACATGG90600.034.3945852
ACATGGG94100.030.5560343
GCAGAGT81300.025.9363299
CATGGGG67350.025.5720064
TAGTACG4700.024.8434874
GAGTACA71450.021.2447431
GTATTAG22950.020.6690751
GTACGGA5301.8189894E-1220.6529466
ACCGCGT4102.0179868E-819.575648
GTATAGA24350.019.1810071
Produced by FastQC (version 0.11.5)