FastQCFastQC Report
Thu 2 Jun 2016
DRR028546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3715330
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48400.067.922831
GTATCAA88300.058.564121
TCAACGC94450.053.677394
ATCAACG95500.053.0113833
CAACGCA101900.049.6819155
TATCAAC106500.047.672042
AACGCAG107950.046.9646076
ACGCAGA122100.041.1067287
CGCAGAG123350.040.690168
GTACATG90100.035.0338331
GCAGAGT138800.033.7605979
TACATGG90600.032.8555642
ACATGGG92950.029.6093943
CATGGGG67900.024.4264874
GTGGTAT26650.024.2893281
GAGTACA71400.023.7345181
CAGAGTA130150.021.8697459
AGTACAT78550.019.9160352
TGGTATC29450.019.4284042
TAGCTCG3252.2250028E-517.8279729
Produced by FastQC (version 0.11.5)