FastQCFastQC Report
Thu 2 Jun 2016
DRR028545_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028545_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075357
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.051.536111
ATCAACG24500.049.741053
TCAACGC24750.048.6489374
GTATCAA27750.047.33411
TATCAAC31800.039.0109372
CAACGCA31350.038.6379285
ACGCAGA31850.037.5703967
CGCAGAG32050.036.8779228
AACGCAG33250.036.2105876
GTACATG45000.032.919111
TACATGG45300.031.734572
GTACGAC1606.5354594E-527.3650263
ACATGGG52850.025.1299133
GCAGAGT42300.024.4903779
ATACCGC1851.7539869E-423.6641487
TACGGGC4154.1836756E-1122.8546568
TCTCGGG39500.021.796694145
TACGACT2053.5104796E-421.358074
GGATAGG13900.021.162916145
CATGGGG36700.020.8779494
Produced by FastQC (version 0.11.5)