FastQCFastQC Report
Thu 2 Jun 2016
DRR028545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075357
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.072.413751
GTATCAA42500.055.612881
TCAACGC44800.051.4087684
ATCAACG45200.050.9538233
CAACGCA49700.046.4860235
TATCAAC52750.044.4846952
AACGCAG52550.044.1027156
ACGCAGA55700.040.8284157
CGCAGAG56100.040.158
GTACATG42800.034.6630971
TACATGG47550.030.7672652
GCAGAGT69250.030.747919
GTGGTAT14450.028.1839031
ACATGGG49300.028.059153
GTACGGA2756.848495E-926.3363386
TGGTATC15300.024.6150072
GAGTACA38800.023.4292961
AGTACGG3604.9130904E-922.1298395
TAGTACG3304.747926E-821.9469474
CATGGGG35800.021.6465574
Produced by FastQC (version 0.11.5)