FastQCFastQC Report
Thu 2 Jun 2016
DRR028544_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028544_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757265
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG15850.048.331283
TCAACGC15850.048.331284
GGTATCA14300.042.855991
CAACGCA19000.040.3184625
AACGCAG20200.037.9233066
GTATCAA21450.037.7540861
CGCAGAG21250.035.015418
GTACATG33100.034.8254971
ACGCAGA21850.034.3897447
TATAGCG1501.080607E-634.0447357
TATCAAC24400.031.3955252
TACATGG34950.031.1033672
TGACGCG1050.00810173727.339935145
ACATGGG39650.027.048453
GCGGGTA1550.001746104423.5298219
CCGGTCG2802.2109634E-723.4457869
GTATAGG8050.022.6575221
GACGAAA3554.64388E-922.237764145
GCAGAGT31600.021.467249
ACCGCGT1800.00415713220.263558
Produced by FastQC (version 0.11.5)