FastQCFastQC Report
Thu 2 Jun 2016
DRR028544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757265
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19320.10994357709281184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19130.10886235143817238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25000.069.55291
GTATCAA41900.053.1330531
TCAACGC45050.049.1985134
ATCAACG45300.048.9273
CAACGCA46700.047.4602365
AACGCAG48850.045.3714036
TATCAAC52050.042.8603132
ACGCAGA55850.039.165997
CGCAGAG56150.038.5697448
GTGGTAT13850.038.3469161
TGGTATC13700.035.422532
GTACATG35800.034.5480271
GCAGAGT67050.031.6514919
TACATGG35800.031.5621722
GTACGAC1153.180743E-431.49183
ACATGGG39150.027.7513943
CCGTCCA2303.1669137E-522.044269
CAGAGTA64400.021.7068469
GGACCGA1750.00368002320.6946136
TAGTACG4551.5643309E-1020.6946134
Produced by FastQC (version 0.11.5)