FastQCFastQC Report
Thu 2 Jun 2016
DRR028543_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028543_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974774
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17900.051.3627174
ATCAACG18100.050.392043
GTATCAA23300.041.964241
CAACGCA22450.041.2779275
AACGCAG23250.039.8576136
GGTATCA15250.037.7996441
ACGCAGA24100.036.6314477
TATCAAC25800.035.0697332
CGCAGAG25100.034.880458
GTACATG40250.028.6432111
CTAGCGT1804.473459E-628.3763183
TACATGG41300.026.8549292
TATACCG1751.2026496E-425.0174876
TCTCGGG29350.024.934538145
ATACCGC1952.5063465E-422.449287
GTATAGG8150.022.382721
GCAGAGT36900.022.3416619
TCGCGGG1952.8071846E-422.07628145
TCTAGCG1700.002980565421.4610792
GTCGTAC1700.002980565421.4610791
Produced by FastQC (version 0.11.5)