FastQCFastQC Report
Thu 2 Jun 2016
DRR028543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974774
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.064.548061
GTATCAA42650.054.2233921
ATCAACG43250.052.413213
TCAACGC43550.052.0521554
CAACGCA46550.048.853145
AACGCAG48750.046.6484876
TATCAAC52250.043.8009262
ACGCAGA54000.041.4426237
CGCAGAG53900.041.3851438
GCAGAGT67850.032.1291479
GTACATG38150.030.8815481
GTGGTAT13250.029.638521
TACATGG40900.025.8530652
GTACGGA2401.6140639E-624.1413356
GTACCGA1851.844612E-423.4888656
ACATGGG44700.021.7113
TGGTATC15900.021.4083542
TATACCG2404.401081E-521.1236675
CAGAGTA63950.020.2719259
TGTACCG2557.0182374E-519.8810985
Produced by FastQC (version 0.11.5)