FastQCFastQC Report
Thu 2 Jun 2016
DRR028542_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028542_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057626
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.050.310911
ATCAACG21400.048.4216583
TCAACGC22050.046.9942634
GTATCAA26400.040.632961
CAACGCA27850.037.2073065
GTACGGA3750.035.027276
GTACATG47800.034.8074381
AACGCAG30700.034.2286076
ACGCAGA30250.033.7711837
TATCAAC31600.033.4846652
CGCAGAG31050.032.193668
TACATGG51200.030.7856882
ACATGGG56100.027.3162773
TAGTACG7300.026.9901924
GCCGTGC7200.024.3214878
AGTACGG5500.023.8822295
GGCGCGT1550.001743143323.5369228
CGGATGC5450.022.7589959
GCAGAGT41500.022.3283319
GAGTACA42500.022.1495971
Produced by FastQC (version 0.11.5)