FastQCFastQC Report
Thu 2 Jun 2016
DRR028542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057626
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24400.072.417981
GTATCAA47200.058.5425341
ATCAACG48150.055.9486243
TCAACGC48650.055.2247544
CAACGCA51100.052.576995
AACGCAG54500.049.562716
TATCAAC58550.046.5054322
ACGCAGA62600.042.5712367
CGCAGAG64200.041.7358678
GTACATG47250.037.8584861
GCAGAGT74900.034.033269
TACATGG51650.032.9488532
TAGATCG1255.1902386E-428.9669485
ACATGGG56250.028.9669463
CATGGGA26400.023.8648134
GTGGTAT15100.023.5965791
TATACCG1952.6363094E-422.2822675
TGGTATC15500.021.4916082
GTACGGA3757.878043E-921.2424286
GAGTACA44500.019.7722131
Produced by FastQC (version 0.11.5)