FastQCFastQC Report
Thu 2 Jun 2016
DRR028541_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028541_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106072
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15650.050.356661
ATCAACG22200.047.9896283
TCAACGC22200.047.9896284
CAACGCA26000.040.974775
GTATCAA27500.040.33281
AACGCAG27850.037.9900056
GTACATG41200.035.2454761
TATCAAC31750.034.474272
ACGCAGA30100.034.4221427
CGCAGAG30450.033.06488
TACATGG42400.031.1501542
GTACGGA3400.030.0452386
ATACCGC2507.3681804E-826.2674887
ACATGGG47650.026.0335583
CTAGCGT1450.001181478325.1622523
GCGTTAC1550.00174280323.5377456
GAGTACA35300.021.911831
GCAGAGT41450.021.4738249
GTATTAG18350.020.6782971
CCGGTCG5002.1827873E-1120.4282959
Produced by FastQC (version 0.11.5)