FastQCFastQC Report
Thu 2 Jun 2016
DRR028541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106072
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24300.071.496041
GTATCAA44150.058.9444121
ATCAACG48000.053.7178573
TCAACGC49550.052.3298264
CAACGCA52450.049.298385
AACGCAG54200.047.5730136
TATCAAC57700.044.68732
ACGCAGA61950.041.2708447
CGCAGAG62650.040.4628948
GTACATG44950.032.3437731
GTGGTAT15750.032.3078351
GCAGAGT77850.031.6322639
TGGTATC14150.031.2236272
TACATGG46100.029.8512552
GTACGGA2654.612957E-927.3315376
ACATGGG47100.026.2957253
CAGAGTA72900.020.0693729
GAGTACA39700.019.9584271
GTATATA18050.019.3310031
TCTGTCG6850.019.0323268
Produced by FastQC (version 0.11.5)