FastQCFastQC Report
Thu 2 Jun 2016
DRR028540_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028540_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990965
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20050.049.498614
ATCAACG20250.048.6493643
CAACGCA23650.041.6553765
GTATCAA25550.041.128231
GGTATCA16350.040.16921
AACGCAG25800.037.901256
ACGCAGA28000.034.1396877
GTACATG40300.033.4992521
TATCAAC29850.033.2478072
CGCAGAG28550.032.96838
TACATGG42500.029.3613222
ACATGGG44600.025.0336993
GCAGAGT37600.022.314069
GAGTACA33950.020.4198361
GTGGTAT12750.020.0320931
GGATAGG12000.019.729063145
GTCTTAC10000.018.9732511
TCTCGGG29550.018.936987145
CATGGGG33250.018.6550224
GTATAGA11000.018.5752121
Produced by FastQC (version 0.11.5)