FastQCFastQC Report
Thu 2 Jun 2016
DRR028540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990965
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24800.068.0043951
GTATCAA41300.058.0850831
TCAACGC43400.054.4056784
ATCAACG43950.053.5600283
CAACGCA46550.050.5684935
AACGCAG48400.048.635616
TATCAAC52600.045.716052
ACGCAGA56150.041.4067967
CGCAGAG56500.040.7657138
GCAGAGT68600.032.0971039
GTACATG41450.031.568121
GTGGTAT14250.029.0777451
TACATGG43550.026.2775752
TGGTATC15350.025.0082822
GCGTTAA2101.5241425E-524.2314531
TAGACCG1550.001819363123.36445
ACATGGG45150.023.1004853
GAGTACA37900.020.7150141
CATGGGA20500.020.1389754
ATATCGC1800.00433282620.1193476
Produced by FastQC (version 0.11.5)