FastQCFastQC Report
Thu 2 Jun 2016
DRR028539_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028539_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896779
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.055.6297571
TCAACGC25550.050.8296664
ATCAACG26400.049.469473
GTATCAA28600.046.4294471
CAACGCA30600.042.4399765
AACGCAG33300.038.9988946
TATCAAC34950.037.36752
GTACATG49000.036.926981
TACATGG47750.035.4489022
ACGCAGA35850.035.4070747
CGCAGAG36550.034.1283578
TATACCG2900.032.705586
ATACCGC3300.030.9488167
ACATGGG53100.030.5032863
GCAGAGT40850.025.535879
GAGTACA41350.025.055381
CATGGGG37450.023.3785514
ACGTCGC1600.002097549622.7995646
AGTACAT40750.022.738632
TTTCGCG2603.0400897E-622.4451929
Produced by FastQC (version 0.11.5)