FastQCFastQC Report
Thu 2 Jun 2016
DRR028539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896779
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.11287556431192036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19410.10233137334396891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.074.0319441
GTATCAA45650.060.0686651
TCAACGC53500.050.501164
ATCAACG53900.050.1263853
CAACGCA58400.046.2639055
TATCAAC61000.045.0044752
AACGCAG62200.043.5539476
ACGCAGA67750.039.772237
CGCAGAG67250.039.6370968
GTACATG49600.038.274211
TACATGG48800.035.326732
GCAGAGT75500.032.907399
ACATGGG51900.031.8213
CAGAGTA70600.026.5730469
GTGGTAT12850.025.4716341
CATGGGG34650.025.2946384
TGGTATC13750.023.1790852
CATGGGA24500.022.1738684
ACTGATC11450.020.8763588
GTCGTAC1750.003591738620.7816181
Produced by FastQC (version 0.11.5)