FastQCFastQC Report
Thu 2 Jun 2016
DRR028538_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028538_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880690
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14200.041.6173024
ATCAACG14650.040.3400463
GCGGGTA1553.2923708E-837.6469579
CAACGCA16900.034.5348055
GTACATG33650.031.6560541
GTATCAA20550.031.598551
AACGCAG18650.031.2934326
GGTATCA14500.031.1969911
CGCAGAG19750.028.0699468
ACGCAGA20350.027.6015267
TACATGG36350.025.6918012
ACATGGG34800.024.3202343
TATCAAC25600.023.9402282
GAGAGGG30600.022.750973145
GACGAAA5400.022.59461145
GTATTAA17800.018.0352291
GAGTACA27300.017.9061051
TCTCGGG35700.017.892517145
GTATATA18000.017.8348391
TAATACT22400.017.2625564
Produced by FastQC (version 0.11.5)