FastQCFastQC Report
Thu 2 Jun 2016
DRR028538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880690
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.065.432871
GTATCAA33300.049.1098181
ATCAACG32900.047.764933
TCAACGC33100.047.2575344
CAACGCA35050.044.6283725
AACGCAG37950.041.408866
TATCAAC42600.037.398862
ACGCAGA43050.036.5032777
CGCAGAG43300.035.9580278
ACGCGCT1555.5456636E-528.0326947
TACGCGC1306.528248E-427.8529996
GCAGAGT53750.027.7545419
GTACGCG1400.001006427925.8634975
GTACATG33750.025.8426781
GTACGGA2051.3024519E-524.7280276
TACATGG35950.023.9713972
GAGTACA29500.023.652621
CCGTGCA3402.5411282E-923.4292859
ACATGGG35350.023.1491133
CAGAGTA52800.021.5333219
Produced by FastQC (version 0.11.5)