FastQCFastQC Report
Thu 2 Jun 2016
DRR028537_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028537_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113373
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG23000.053.9256253
TCAACGC22900.053.841224
GTATCAA28200.047.3452151
GGTATCA16400.043.1521071
CAACGCA29950.041.6535955
GTACATG52750.039.141561
AACGCAG32650.037.7621546
TATCAAC33750.037.397832
TACATGG53600.036.8874472
ACGCAGA33350.034.999077
CGCAGAG32800.034.9170278
ACATGGG56800.033.396363
GAGTACA41800.026.5302541
CATGGGA23900.025.6415944
CATGGGG38450.024.6666774
GTACGGA3601.70985E-1024.318236
AGTACAT42700.023.9207232
CCGTGCA8650.023.612559
GCAGAGT41950.023.3009599
GAGAGGG46100.022.573635145
Produced by FastQC (version 0.11.5)