FastQCFastQC Report
Thu 2 Jun 2016
DRR028537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113373
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.067.600621
GTATCAA40000.050.370331
TCAACGC40600.048.1635634
ATCAACG42050.046.5027543
CAACGCA45000.043.454245
AACGCAG47700.040.8427356
GTACATG48800.040.0947461
TATCAAC51100.039.684242
CGCAGAG52450.036.591598
ACGCAGA53100.036.2800647
TACATGG50850.035.4641272
ACATGGG54550.030.5361293
GCAGAGT64600.027.8035379
CATGGGA21350.026.1200354
TCTATAC15000.022.2099443
GTACGGA3655.7625584E-921.826336
GAGTACA39150.021.7374741
TAGATCG1700.003112864221.3010985
GTACTAG6200.021.1171761
CATGGGG38650.020.4248054
Produced by FastQC (version 0.11.5)