FastQCFastQC Report
Thu 2 Jun 2016
DRR028536_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028536_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584576
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14350.049.832354
ATCAACG14550.049.6488723
CAACGCA17150.041.695125
GTATCAA18550.040.9097331
GGTATCA12500.039.695031
AACGCAG18200.039.6905486
TATCAAC21600.034.119672
CGCAGAG20950.033.433568
ACGCAGA21250.033.3049057
GACGAAA4850.032.54662145
TTACGCA1153.0455724E-431.7255654
GTACATG30400.031.6838191
TACATGG34400.027.7875392
TGAACCG2051.22827205E-524.9153755
ACATGGG35850.023.6105483
GCAGAGT29200.022.4874979
AGTACGG3004.2853935E-721.8899365
GTACGGA2053.5122226E-421.3560356
GAGTACA29150.020.5263861
TATACTG11500.020.303715
Produced by FastQC (version 0.11.5)