FastQCFastQC Report
Thu 2 Jun 2016
DRR028536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584576
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.072.7027741
GTATCAA33100.056.8882751
TCAACGC35750.051.6631974
ATCAACG36300.050.880423
CAACGCA38200.048.5393265
AACGCAG40550.045.3690836
TATCAAC42200.044.4533462
ACGCAGA43900.041.5777
CGCAGAG44050.041.2709928
GTACGGA2252.0008883E-1135.410116
GCAGAGT55400.032.423449
GTACATG30700.030.5493741
GTGGTAT10950.027.8859961
CGGATGC3204.1836756E-1127.1611679
TACATGG33250.025.4865682
TGGTATC10850.025.367112
AGTACGG3601.8735591E-1024.143265
ACATGGG34800.023.7269973
ACGGATG4053.274181E-1123.2490638
GCTATAC5450.022.5927723
Produced by FastQC (version 0.11.5)