FastQCFastQC Report
Thu 2 Jun 2016
DRR028535_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028535_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3504710
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30050.050.2574461
TCAACGC43950.047.1448254
ATCAACG44150.047.0965043
GTATCAA51200.042.8914831
CAACGCA51600.040.1553275
GTACATG88450.038.7681051
AACGCAG54400.037.9538466
TACATGG90200.035.427632
TATCAAC62650.033.8880422
ACGCAGA61500.032.9761667
ACATGGG94100.032.176073
CGCAGAG62950.031.9834218
GTACGGA5250.026.4035636
CATGGGG63150.025.7635734
GAGTACA72500.023.7491781
GCAGAGT73300.023.3846769
GACGAAA12650.022.126762145
CATGGGA41750.022.0185454
AGTACAT72900.021.7173482
AGTACGG8850.018.9609055
Produced by FastQC (version 0.11.5)