FastQCFastQC Report
Thu 2 Jun 2016
DRR028535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3504710
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43900.076.331761
GTATCAA83300.054.451331
ATCAACG87700.050.8720933
TCAACGC88700.050.298564
CAACGCA95950.046.4225125
AACGCAG101000.044.029686
TATCAAC104000.043.1774332
ACGCAGA112400.039.177417
CGCAGAG113350.038.5934758
GTACATG86650.034.3102261
TACATGG86350.032.711522
GCAGAGT130850.032.158889
ACATGGG85550.030.9855753
GTGGTAT23100.029.2644161
TGGTATC26700.024.1422212
CATGGGG60700.023.2672144
GAGTACA69250.021.4130861
CAGAGTA126600.020.8240959
GTATAGG17600.019.824281
CATGGGA41900.018.4955924
Produced by FastQC (version 0.11.5)