FastQCFastQC Report
Thu 2 Jun 2016
DRR028534_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028534_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987643
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16750.051.4048964
ATCAACG17700.048.2336243
CAACGCA20350.041.950445
GGTATCA15700.038.575871
GACGAAA6050.037.952084145
AACGCAG22700.037.6075556
GTATCAA25550.036.5558051
GTACATG39700.036.3925061
CGCAGAG25050.033.201428
ACGCAGA25650.032.9961667
TACATGG41650.032.5863152
TATCAAC28350.030.8862722
ACATGGG45200.027.2825873
GCAGAGT33400.022.7161779
TATACTG15150.022.1544135
GTACGGA2353.532879E-521.7342826
CATGGGA21850.020.7051094
AGTACGG3951.31367415E-820.319395
GAGTACA33500.019.6035611
ATGGGCG3751.6924969E-719.4573555
Produced by FastQC (version 0.11.5)