FastQCFastQC Report
Thu 2 Jun 2016
DRR028534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987643
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.063.071481
GTATCAA35250.055.473181
TCAACGC36050.052.8321764
ATCAACG36350.052.3961523
CAACGCA39100.048.525795
AACGCAG40800.046.6813776
TATCAAC46250.041.6502842
ACGCAGA46250.040.7108047
CGCAGAG46200.039.971118
GTACATG39950.035.663931
GTACGGA2103.8380676E-1034.4848826
TACATGG41400.031.8360442
GCAGAGT56600.030.9632849
ACATGGG42650.029.2050173
AGTACGG3151.0495569E-925.2889145
GTGGTAT11800.023.4094661
GAGTACA33550.021.88361
GTCTCGC5051.8189894E-1221.5918461
AGTACAT33100.021.222272
GAACGGT2053.6962546E-421.1955856
Produced by FastQC (version 0.11.5)