FastQCFastQC Report
Thu 2 Jun 2016
DRR028533_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028533_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636337
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.047.450681
TCAACGC15900.045.8839074
ATCAACG16800.043.4258463
CAACGCA18050.040.0143285
GTATCAA20850.038.139761
AACGCAG19450.037.508056
GTACATG33100.034.1634141
ACGCAGA22150.032.277247
GACGAAA5150.032.051296145
TATCAAC23450.031.7332732
CGCAGAG22500.031.1257118
TACATGG34350.031.0087072
ACATGGG36950.027.6421073
TCTACGC1100.009382497526.5292433
GTCTCGC4900.022.333291
GTACGGA2104.1379986E-420.843766
AGTACCG2205.6615716E-419.8969335
GCAGAGT30500.019.8515919
CATGGGG23900.019.536184
GAGTACA28900.018.9330671
Produced by FastQC (version 0.11.5)