FastQCFastQC Report
Thu 2 Jun 2016
DRR028533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636337
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17280.1056017189613142No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17120.10462392526722795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.070.139111
GTATCAA36550.057.716961
TCAACGC40000.052.15114
ATCAACG40400.051.276183
CAACGCA42000.049.6677135
AACGCAG44150.047.0849576
TATCAAC46950.044.585462
ACGCAGA49950.040.892597
CGCAGAG50550.040.120648
GTACATG34300.037.962241
TACATGG37550.032.0205762
GCAGAGT60900.031.993819
GTGGTAT12600.031.1756921
TGGTATC12550.031.1659972
ACATGGG39500.028.6060923
GTACGGA2054.0750638E-728.266186
TAGACCG1100.00971763526.3389425
TAACGCA1550.001818868123.3651894
ATACCGC2502.2993445E-623.1782677
AGTACCG2202.246142E-523.0465745
Produced by FastQC (version 0.11.5)