FastQCFastQC Report
Thu 2 Jun 2016
DRR028532_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028532_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800792
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17650.061.608854
ATCAACG18300.059.819363
CAACGCA20400.053.3037385
GGTATCA12400.050.614891
AACGCAG22850.047.2690736
GTATCAA24050.047.0346761
TATCAAC28100.039.736232
ACGCAGA25550.039.7020727
CGCAGAG25800.039.0345048
GTACATG37100.032.8506621
TACATGG39550.029.523992
GCAGAGT33300.027.1717539
ACATGGG43050.025.2589133
GTACGGA2901.1112206E-825.1653826
CTAGCGT1600.002094000322.806133
AGTACGG3253.7249265E-822.4552655
GTATTAG17400.021.811
GACGAAA6150.020.993883145
CGTTATA1850.004859507519.724222
GAGAGGG35500.019.195112145
Produced by FastQC (version 0.11.5)