FastQCFastQC Report
Thu 2 Jun 2016
DRR028532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800792
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.075.127881
GTATCAA39700.060.05311
TCAACGC41650.057.03014
ATCAACG41550.056.993073
CAACGCA43850.054.0036935
AACGCAG45700.051.8175476
TATCAAC48400.048.777282
ACGCAGA52550.044.64967
CGCAGAG53250.043.5186738
GCAGAGT64600.034.639479
GTACATG38800.034.4697571
TACATGG41400.030.6113872
GTACGGA2859.094947E-1230.4917036
ACATGGG44850.027.12643
CTATGCG1400.001006410925.8634999
AGTACGG3601.8735591E-1024.1392655
TCTGTCG5900.023.3209868
GAGTACA36700.023.1724471
AGTACAT34700.022.5392572
GTGGTAT12600.022.4981161
Produced by FastQC (version 0.11.5)