FastQCFastQC Report
Thu 2 Jun 2016
DRR028531_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028531_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866022
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19950.051.206454
ATCAACG20700.049.352493
GTATCAA23650.046.2819331
GGTATCA14350.046.2744061
GTACATG44500.040.1751441
CAACGCA25800.039.878515
TATCAAC27450.038.2799642
AACGCAG27450.037.215626
ACGCAGA27650.035.6239977
CGCAGAG28100.035.3112378
TACATGG48800.034.2426222
GTCGTAC1501.0785516E-634.0532151
ACATGGG54550.029.4292563
GTACGGA2456.0539605E-826.8050086
TAATCCG1500.001439633424.3230635
GCAGAGT39000.023.3837369
GGACCGC2202.1216118E-523.217476
TCTGTCG5400.022.9686538
CATGGGG40450.022.7295884
TAGTACG6000.021.8907574
Produced by FastQC (version 0.11.5)