FastQCFastQC Report
Thu 2 Jun 2016
DRR028531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866022
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23350.073.5196151
GTATCAA41300.059.1789631
ATCAACG44200.054.4021763
TCAACGC44400.054.320254
CAACGCA47850.050.5551035
AACGCAG50900.047.525776
TATCAAC51700.046.510182
ACGCAGA57100.041.731147
CGCAGAG58650.040.5047848
GTGGTAT11350.032.685291
GTACATG45500.032.453591
GCAGAGT71500.031.6045079
TACATGG48050.028.4884552
ACATGGG49700.026.6682913
GCGTTAC2101.5681924E-524.1423346
TGGTATC14700.023.156932
CTAGCGT2303.168449E-522.0433
GCCGTGC5750.021.4131988
CATGGGA21900.021.165884
CAGAGTA67050.021.0637829
Produced by FastQC (version 0.11.5)