FastQCFastQC Report
Thu 2 Jun 2016
DRR028530_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028530_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953634
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.052.150921
TCAACGC23250.051.7833634
ATCAACG24100.050.5625153
GTATCAA29000.044.032091
CAACGCA28650.041.5116165
GTACATG52050.041.0748671
AACGCAG30700.038.977336
TACATGG52600.037.5935062
TATCAAC34200.035.843672
ACGCAGA33400.034.5139477
ACATGGG53800.033.9068153
CGCAGAG34800.033.123738
GTACGGA3300.030.9542836
CATGGGG37900.026.1835774
GCAGAGT40700.025.8111239
GTACTAG4800.024.3224891
CATGGGA23750.023.9640524
GAGTACA44400.022.5147381
AGTACAT40500.022.3406562
TATACCG3706.188202E-921.69196
Produced by FastQC (version 0.11.5)