FastQCFastQC Report
Thu 2 Jun 2016
DRR028530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953634
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25350.071.708661
GTATCAA46500.056.1372451
TCAACGC49200.050.9324154
ATCAACG49700.050.4200133
CAACGCA52850.047.5518885
AACGCAG56650.044.3621756
TATCAAC57650.044.0951772
ACGCAGA63650.039.142037
CGCAGAG65450.038.0655488
GTACATG53700.037.7780461
TACATGG55000.035.2903022
ACATGGG56300.032.6744733
GCAGAGT73550.032.494859
GTGGTAT12600.031.1625351
CATGGGG38550.029.3078174
GTATAGG10250.025.5380781
TGGTATC15200.025.2531492
GAGTACA45550.024.9026621
AGTACAT43150.023.4979782
CAGAGTA72250.021.6520589
Produced by FastQC (version 0.11.5)