FastQCFastQC Report
Thu 2 Jun 2016
DRR028529_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028529_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963557
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC21100.051.876474
ATCAACG21400.051.831223
GTATCAA25500.045.2145771
CAACGCA23950.045.092735
GGTATCA16200.044.14411
AACGCAG27150.039.777936
TATCAAC30900.037.0768432
ACGCAGA28850.036.67527
CGCAGAG28300.035.8399548
GTACATG44800.034.368931
TACATGG47800.029.769282
GACGAAA6050.028.455397145
ACATGGG48500.027.0827263
GCAGAGT39600.022.1112799
TACGCTA2053.5111635E-421.3573685
GTATTAG15700.020.9157981
CATGGGA20700.020.7990384
GAGTACA38050.020.328851
TATAGCG1800.00414935620.2702482
GCGTAGC1850.004862251719.7224041
Produced by FastQC (version 0.11.5)