FastQCFastQC Report
Thu 2 Jun 2016
DRR028529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963557
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.068.1474461
GTATCAA40900.053.348181
TCAACGC42100.050.4042934
ATCAACG42350.049.9357343
CAACGCA44400.047.956385
CCTAGCG803.759216E-545.2649463
AACGCAG48100.044.417996
TATCAAC49750.043.5271342
ACGCAGA53150.039.3800777
CGCAGAG53750.038.536268
GTACATG42300.033.1847531
GCAGAGT63850.031.6464719
TACATGG44200.028.5107732
ACATGGG45900.026.0347393
GTGGTAT13700.024.4207461
GAGTACA39700.022.7170961
TGGTATC14450.021.5517542
AGTACAT37500.021.4374792
CATGGGG33100.020.3487114
GTCTAGC5403.6379788E-1220.2031541
Produced by FastQC (version 0.11.5)