FastQCFastQC Report
Thu 2 Jun 2016
DRR028528_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028528_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695145
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.049.419451
TCAACGC17750.048.0961384
ATCAACG18100.046.7629783
GTATCAA20950.043.187731
CAACGCA20050.041.8497735
GTACATG38250.037.9615861
AACGCAG22000.037.808716
TATCAAC24900.034.28542
TACATGG38800.033.6623152
ACGCAGA25000.032.3960957
GACGAAA5250.030.065783145
CGCAGAG25550.029.9843778
CTAGCGT1306.246144E-428.0639863
ACATGGG43000.026.3018283
GCGTTAC1550.001743055223.536836
TCTAGCG1600.002096160322.801992
GTATATA15900.022.0275821
GAGTACA34900.021.5344871
ATACCGA1750.003526867120.8469076
GTATAGG7750.020.7130341
Produced by FastQC (version 0.11.5)