FastQCFastQC Report
Thu 2 Jun 2016
DRR028528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695145
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17160.10123027823578513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.074.156491
GTATCAA38150.059.676941
TCAACGC42900.052.1732524
ATCAACG43550.051.3945473
CAACGCA46450.048.341785
AACGCAG47650.047.1243636
TATCAAC49100.045.8802342
CGCAGAG53500.041.700738
ACGCAGA53700.041.545427
GTACATG37350.037.0015371
TAGACCG1001.3992532E-436.2173545
AATACCG1458.7647277E-734.968485
TATAACG1051.8641015E-434.492722
GCAGAGT65100.033.2687849
TACATGG41250.031.2566742
GTGGTAT10950.030.5562761
ACATGGG41050.029.2915043
TGGTATC12350.026.3932132
CAGAGTA63050.022.1727189
GTACGGA2053.6902787E-421.2004036
Produced by FastQC (version 0.11.5)