FastQCFastQC Report
Thu 2 Jun 2016
DRR028527_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028527_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647457
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA400.00595403254.5987936
TCAACGC16200.051.230074
ATCAACG16950.048.9632533
CAACGCA17650.047.019925
GTATCAA20600.042.7615361
AACGCAG19550.042.0778476
GGTATCA13900.041.3758931
ACGCAGA22500.036.23527
CGCAGAG22550.035.508148
TATCAAC24800.035.226112
GTACATG33950.031.9507881
TACATGG34450.030.219132
GTACGGA1952.5168083E-729.8660076
ACATGGG36400.025.600223
AGTACGG3201.1841621E-925.0244465
ATACCGT2651.3238059E-724.7239826
TCTCGGG22250.024.275045145
GAGTACA30150.023.6632211
GCAGAGT30550.023.349099
GTCTCGC3152.744855E-823.111311
Produced by FastQC (version 0.11.5)