FastQCFastQC Report
Thu 2 Jun 2016
DRR028527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647457
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20450.077.848861
GTATCAA35800.053.810161
ATCAACG38350.048.5368273
TCAACGC38350.048.5368274
CAACGCA40100.046.418645
AACGCAG43300.042.988166
TATCAAC45850.040.755292
ACGCAGA49350.037.571327
CGCAGAG49400.036.9468358
GTACATG31850.030.8124181
GCAGAGT58850.030.0294239
GACCGAT1000.00610534228.9710127
TGGTATC11500.027.0815962
TACATGG33950.025.8136392
GTGGTAT13200.024.782211
ACATGGG34300.024.072123
CGCTAAT1650.002616718221.9477352
TCTGTCG4251.4897523E-920.4501238
GGACCGA1800.004333249320.1187576
CAATACG2205.945508E-419.7529624
Produced by FastQC (version 0.11.5)