FastQCFastQC Report
Thu 2 Jun 2016
DRR028526_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028526_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747601
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19700.051.7806554
ATCAACG20200.050.4989553
GGTATCA13950.048.5751841
GTATCAA24250.044.1683651
GTACATG40800.042.6818731
CAACGCA24650.041.0857285
TACATGG43350.037.313812
TATCAAC28400.037.2010652
AACGCAG27050.037.171066
ACGCAGA27500.035.5019877
CGCAGAG26900.035.2073948
ACATGGG46700.033.7009853
CATGGGA20000.029.145114
GAGCGAC9550.024.098936145
GCAGAGT36400.023.414759
CATGGGG31000.023.034044
GTACTAA10300.021.9295731
TCTATAC9500.020.7083663
GAGTACA34350.020.3633981
TATACCG2205.711592E-419.871096
Produced by FastQC (version 0.11.5)