FastQCFastQC Report
Thu 2 Jun 2016
DRR028526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747601
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.069.2571951
GTATCAA38450.051.8331221
ATCAACG40000.048.1622733
TCAACGC40100.048.0421684
CAACGCA43650.044.1349565
AACGCAG47400.040.643276
TATCAAC48150.040.160612
GTACATG44700.037.751451
ACGCAGA50750.037.6749957
CGCAGAG51300.036.9887168
ATACCGC2252.0008883E-1135.4075167
TACATGG46750.034.8566972
ACATGGG50200.030.7299023
GCAGAGT60950.029.2312039
TATACCG2752.1645974E-1028.9697886
GTGGTAT10600.028.1339781
CATGGGA20850.023.9678124
CATGGGG35100.023.316144
TATACTG11550.022.573865
CAGAGTA60000.022.4515849
Produced by FastQC (version 0.11.5)