FastQCFastQC Report
Thu 2 Jun 2016
DRR028525_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028525_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495205
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14750.050.9285474
ATCAACG14900.050.4158483
CAACGCA16800.044.7140545
GTATCAA18450.041.9011151
GGTATCA11200.041.6752361
AACGCAG19150.039.226956
ACGCAGA20500.036.6424647
TATCAAC21600.035.790542
CGCAGAG21100.034.562428
GTACATG32750.031.1768931
TGTACCG1555.284037E-528.2316115
GTACGAC1409.656459E-426.0470223
TACATGG37000.025.4275252
TCTCGGG19150.024.371504145
ACATGGG38050.023.5758083
GAGCGAC5850.023.320374145
GCAGAGT28800.021.7767249
GTCTAGC4451.05501385E-1021.305881
GTCTCGC3508.211828E-820.8376181
GAGTACA29800.019.8237081
Produced by FastQC (version 0.11.5)