FastQCFastQC Report
Thu 2 Jun 2016
DRR028525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495205
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15190.10159142057443629No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15020.10045445273390605No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.077.2906651
GTATCAA33900.059.4682041
ATCAACG36900.053.187253
TCAACGC37250.052.6875044
CAACGCA38400.051.109625
AACGCAG40700.048.3993036
TATCAAC42500.046.179052
ACGCAGA46350.042.1877
CGCAGAG46700.041.7157448
GTACATG32400.033.021
GCAGAGT60700.031.2591539
TACATGG36800.026.3706952
ACATGGG36700.024.4692273
TGGACCG1500.00150413624.1403375
ACCGTCC1500.00150413624.1403378
CAGAGTA55600.021.3615939
GTGGTAT10550.020.6953871
TCACCGG2557.01901E-519.880278145
GTATATA13300.019.152311
CCGTGCA2701.0886885E-418.7758189
Produced by FastQC (version 0.11.5)