FastQCFastQC Report
Thu 2 Jun 2016
DRR028524_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028524_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3363230
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG33850.045.90623
TCAACGC33700.045.6775744
GGTATCA25950.044.700281
GTATCAA41100.039.050931
CAACGCA41000.037.544175
GTACATG75200.036.6711431
AACGCAG44550.034.5524336
ACGCAGA47700.032.1153037
TACATGG82050.032.009172
TATCAAC49650.031.8853342
CGCAGAG48350.030.9273158
ACATGGG85900.027.8567963
TCTCGGG47300.023.823103145
GAGTACA67800.023.3496571
AGTACAT63750.023.3453542
GTATACG4505.456968E-1222.696871
GTCGTAC1952.5082432E-422.4474541
CATGGGG59200.021.8123264
GCAGAGT63400.020.2486069
CATGGGA42050.019.0843414
Produced by FastQC (version 0.11.5)