FastQCFastQC Report
Thu 2 Jun 2016
DRR028524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3363230
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40300.076.1527941
GTATCAA77650.056.1937751
ATCAACG80450.052.8490523
TCAACGC81150.052.303924
CAACGCA87650.048.4251375
AACGCAG92300.045.985526
TATCAAC95800.044.6834952
ACGCAGA103650.040.6704377
CGCAGAG103950.040.622748
GTACATG78400.036.1765331
GCAGAGT121100.032.955869
GTGGTAT21350.032.7003061
TACATGG82750.032.3861162
ACATGGG83400.029.701973
TGGTATC23000.027.0829372
ATACCGC8350.021.6859637
CAGAGTA119000.021.4858689
CATGGGA38650.021.1764974
GAGTACA69450.021.0475831
CATGGGG61050.020.9996834
Produced by FastQC (version 0.11.5)