FastQCFastQC Report
Thu 2 Jun 2016
DRR028523_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028523_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847827
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG20950.050.774663
GGTATCA14750.050.3832631
TCAACGC21300.050.2823874
CAACGCA24650.042.855395
GTATCAA26500.042.340211
AACGCAG26500.039.038836
GTACATG49550.038.083271
TATCAAC30200.034.9815642
ACGCAGA29400.034.9383437
ACGTCGC1051.8019661E-434.6924556
TACATGG51400.033.8776252
CGCAGAG30550.032.905968
TACGTCG1153.0739943E-431.675725
ACATGGG53350.030.8639983
TAGCGTG1952.5014742E-729.8872477
TCTCGGG27900.025.26408145
CATGGGG35000.024.355444
GCAGAGT40350.023.6488849
CATGGGA24850.022.5757664
GAGTACA42550.022.0886061
Produced by FastQC (version 0.11.5)