FastQCFastQC Report
Thu 2 Jun 2016
DRR028523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847827
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.078.358121
GTATCAA43950.059.7026941
ATCAACG45600.055.586743
TCAACGC45700.055.4651074
CAACGCA48200.052.5882845
AACGCAG50950.050.0341456
TATCAAC54200.047.568422
ACGCAGA55350.045.925887
CGCAGAG56800.044.4984748
GTACATG45050.037.5929761
GCAGAGT68200.035.7860349
TACATGG48900.033.7671172
ACATGGG49600.031.684443
GTGGTAT12650.030.453061
TTTCGCG1952.6341877E-729.711429
TGGTATC12450.027.3398742
TAGATCG1400.001006114125.8648515
CATGGGG33000.024.7989064
CATGGGA22150.022.5602234
GAGTACA39700.020.8718011
Produced by FastQC (version 0.11.5)