FastQCFastQC Report
Thu 2 Jun 2016
DRR028522_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028522_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710671
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16300.054.9906434
ATCAACG16950.052.8818633
CAACGCA19450.046.0847055
GTATCAA20900.044.6308331
GGTATCA12250.042.831941
AACGCAG21750.041.5464326
ACGCAGA22350.038.8008087
CGCAGAG22050.038.3326878
TATCAAC24850.036.9500852
GTACATG34250.035.107081
TACATGG37850.029.4575672
GGACCGT1504.248822E-529.1495156
ACATGGG38650.028.8478373
TACACGC3001.617991E-824.2912645
TCTCGGG21850.024.019451145
GCAGAGT32550.023.728749
GTATAGG7400.023.6347431
GTATATA16450.021.7070851
GAGCGAC6650.020.541098145
GAGTACA28550.020.1647241
Produced by FastQC (version 0.11.5)