FastQCFastQC Report
Thu 2 Jun 2016
DRR028522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710671
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.072.362821
GTATCAA41500.062.3559881
TCAACGC47200.054.6237954
ATCAACG47400.054.3933143
CAACGCA49700.051.8761185
AACGCAG52050.049.812256
TATCAAC54000.048.2817042
ACGCAGA59750.043.1505137
CGCAGAG59500.043.088388
ACTATCG800.002047732736.2112778
GCAGAGT70700.034.9308249
GTACATG37000.032.2194671
GTCGTAC1000.00599834129.0761051
TACATGG39750.028.0580462
GTGGTAT13300.026.234081
ACATGGG41050.025.0523823
CGTACAC2351.343813E-624.6544883
TGGTATC13500.023.604392
CAGAGTA67950.021.955929
GAGTACA32450.021.504671
Produced by FastQC (version 0.11.5)